ABSTRACT
Objective To evaluate the effect of Ji Tang Zhi on glucose metabolism in insulin resistance (IR) HepG 2 cell line,and to explore the related mechanism.Methods The HepG2 cells were incubated in culture medium addition of 10-7 mol/L insulin for 24 h to establish the IR cell model.Effect of Ji Tang Zhi on rate of glucose absorption in HepG2 cell was detected by the method of glucose oxidase-peroxidase (GOD-POD).We performed an MTT assay to determine cytotoxicity effects of Ji Tang Zhi on HepG2 cell line.The expression of p-IRS-1 Ser307,PI3K and GLUT-4 were detected by Western blotting.Results Incubated with 10-7 mol/L insulin for 24 h,the insulin resistance cell model had been built.Compared with model group,the rate of glucose absorption of cell treated with JTZ (30 ～ 120 μg/mL) was significantly improved.According to model cells,the expression of GLUT-4 and PI3K decreased significantly compared to control cells.While the expression of p-IRS-1 Ser 307 was inhibited and GLUT-4 and PI3K expression were increased in IR cells after treated with JTZ (30 ～ 120 μtg/mL).Conclusion JTZ exert beneficial effects on hyperglycosemia in IR cell line possibly through regulating the levels of GLUT-4,p-IRS-1 Ser307 and PI3K in HepG2 cell.
ABSTRACT
Objective To evaluate the therapeutic effect of mini-incision surgery for the treatment of varicose vein of lower extremities. Methods 106 limbs of 95 paitnets with varicose vein underwent saphenectomy combined with ligation of communicating veins by way of mini-incision from November 1997 to April 2001. The ascending venography and StethoDoppler examination were performed preoperatively in all of the patients. Results Venous reflux in deep vein of lower extremities were at grade 0 in 62 limbs, at grade Ⅰ in 28 limbs and at grade Ⅱ in 16 limbs demonstrated by ascending venography with Valsalva test. StethoDoppler examination showed that valve insufficiency of great saphenous vein at saphenofemoral junction was in 99 limbs, the insufficiency of small saphenous vein at saphenopopliteal in 5 limbs and incompetent perforating vein in all of the limbs. Postoperative course was uneventful, the average hospitalization days after operation were 2.8 days and no recurrent varicose veins occurred in all the patients during the follow-up period lasting 1-30 months. Conclusions With the help of preoperative StehoDoppler examination, the mini-incision surgery for the treatment of varicose vein of lower extremities is safe, simple, effective and lower costs.
ABSTRACT
ObjectiveTo evaluate a new cross-linking reagent—polyepoxy compound(PC)in vivo. MethodsTo reconstruct abdominal aota with jugular homografts of SD rats (diameter 0.8～1.1mm) pret4reated by glycero polyglycidyl ether(a kind of PC) or glutaraldehyde(GA). Grafts were explanted at different elapsed time, and their histological changes were studied. ResultsThere was no difference between the patencies of the PC and GA prepared homografts ( P >0.05), but the inci-dence of aneurysms in GA is higher than that in PC group ( P < 0.05). The healing procedures of endothelial cells in two experimental groups were basically same at the end of second month after transplantation. The endothelial cells cover the whole inner wall of the grafts. ConclusionCompaired PC graft with GA graft, it shows that PC are more unbreakable and compliant, lower rate of aneurysms and less calcification. PC might be a potential vessel-crosslink-reagent.
ABSTRACT
OBJECTIVE To evaluate the safety and clinical efficacy of cefoperazone/sulbactam in the treatment of biliary tract infections.METHODS In this prospective multicenter study,159 hospitalized patients with biliary tract infections received cefoperazone/sulbactam,and the clinical and bacteriological efficacy as well as the side effects were evaluated.RESULTS The clinical effective rate of cefoperazone/sulbactam in the treatment of biliary tract infections was 86.78%.After treatment,the body temperature reduced to normal rapidly,the average time of defervescence was 3.09?1.81 days.Pathogen eradication rate was 85.71%.No adverse reactions were reported during the study period.CONCLUSIONS Cefoperazone/sulbactam can be used as one of antibiotics of choice in the initial empirical therapy for biliary tract infections.
ABSTRACT
Objective To evaluate the clinical effectiveness of extended profundaplasty for treatment of critical leg ischemia. Methods Twenty-five legs (stage Ⅲ and Ⅳ in Fontaine′s classification) of 23 patients with critical leg ischemia caused by extensive occlusion of the distal arteries were reviewed during past 12 years. All patients underwent extended profundaplasty and were evaluated by clinical observation of API, tcPO 2, PSaO 2, Treadmill test, NIRS and ultrasound perioperatively. Results Fourteen legs in 13 patients had a favorable outcome, seven legs in 6 patients had an improvement on their symptom and sign, three legs in 3 patients underwent below knee and one case medio-tarsal amputation during the periods of 7 months to 7 years follow-up. Conclusion The extended profundaplasty is simple, less invasive, safe and effective for the treatment of limb-threatening ischemia.